Determination Pantoprazole on Obelisc R Column

Separation type: Liquid Chromatography Ion Exclusion
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High Performance Liquid Chromatography (HPLC) Method of Pantoprazole.

Pantoprazole, a drug from the proton pump inhibitors (PPIs) group, is widely used for treatment of gastroesophageal disease and peptic ulcer. It can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Pantoprazole can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV at 290nm, ELSD, CAD or LC/MS.



Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN -35%
Buffer Ammonium Acetate  pH 5.5 – 10 mM
Flow Rate 1.0 ml/min
Detection UV 290 nm



Class of Compounds
Analyzing Compounds Pantoprazole


Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Pantoprazole sodium
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.