Determination Pantoprazole on Obelisc R Column

Separation type: Liquid Chromatography Ion Exclusion View on

High Performance Liquid Chromatography (HPLC) Method of Pantoprazole. Pantoprazole, a drug from the proton pump inhibitors (PPIs) group, is widely used for treatment of gastroesophageal disease and peptic ulcer. It can be retained on an Obelisc R column, which has both positive and negative ion-pairs embedded in the stationary phase, allowing for the fine tuning and separation of a wide range of compounds with different ionic properties. Pantoprazole can be determined isocratically using a simple MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate (AmAc) buffer and detected by UV at 290nm, ELSD, CAD or LC/MS.



Column Obelisc R, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN -35%
Buffer Ammonium Acetate  pH 5.5 - 10 mM
Flow Rate 1.0 ml/min
Detection UV 290 nm


Class of Compounds  Drug
Analyzing Compounds Pantoprazole
Application Analytes:
Pantoprazole sodium