Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method of N-Acetyl-L-Cysteine.
N-Acetyl-L-Cysteine (NAC) is the N-acetyl derivative of the naturally occurring amino acid L-cysteine. It can be retained on Primesep SB, a reverse-phase (RP) mixed-mode column with embedded strong basic ion-pairing groups, using an isocratic analytical method with a simple mobile phase of water, acetonitrile (MeCN, ACN), and phosphoric acid (H3PO4) buffer. UV detection at 200 nm.
| Column | Primesep SB, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 20% |
| Buffer | H3PO4 0.3% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Column | Primesep SB, 3.2×100 mm, 5 µm, 100A |
| Mobile Phase | MeCN – 50% |
| Buffer | Na2HPO4 pH 4.0 – 50 mM |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 200 nm |
| Class of Compounds |
Nutritional supplement, Hydrophilic, Ionizable |
| Analyzing Compounds | N-Acetyl-L-Cysteine |
Application Column
Primesep SB
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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