CAS Number | 59277-89-3 |
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Molecular Formula | C8H11N5O3 |
Molecular Weight | 225.208 |
InChI Key | MKUXAQIIEYXACX-UHFFFAOYSA-N |
LogP | -1.56 |
Synonyms |
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Applications:
HPLC Method for Simultaneous Determination of Acyclovir and Hydrocortisone on Primesep 100 Column
August 31, 2022
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir and Hydrocortisone
Acyclovir is used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. It can also be used to treat chickenpox in children. Hydrocortisone, or cortisol, is a common corticosteroid used to treat a wide variety of ailments, including high blood calcium, arthritis, dermatitis, and asthma, among others. Both of these drugs can be separated, retained, and analyzed on a Primesep 100 mixed-mode column using an isocratic analytical method with a simple isocratic mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be UV detected at 254 nm with high resolution and peak symmetry.Column | Primesep 100, 3.2×150 mm, 5 µm, 100A |
Mobile Phase | MeCN – 35% |
Buffer | H2SO4 – 0.1% |
Flow Rate | 0.5 ml/min |
Detection | UV 254 nm |
Class of Compounds | Drug |
Analyzing Compounds | Acyclovir and Hydrocortisone |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsHydrocortisone
HPLC Separation of Antiviral Drugs on Primesep 100 Column
April 1, 2021
Separation type: Liquid Chromatography Mixed-mode
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High Performance Liquid Chromatography (HPLC) Method for Analysis of Acyclovir, Oseltamivir, Valacyclovir
Unlike antibiotic drugs, which actively destroy their targets, antiviral drugs generally prevent the reproduction of their viral targets. Acyclovir, Valacyclovir, and Oseltamivir are 3 antiviral drugs commonly prescribed to treat viral infections. Acyclovir and Valacyclovir are used to treat viral infections caused by a group of viruses called herpes simplex viruses. These infections include oral and genital herpes, as well as shingles. They can also be used to treat chickenpox in children. Oseltamivir is generally used to help treat (or if taken early enough, prevent) influenza (the flu) infections.
Acyclovir, Valacyclovir, and Oseltamivir can be retained on a Primesep 100 mixed-mode column with great peak shape using acetonitrile (ACN), water and a gradient of sulphuric acid (H2SO4) as a buffer. This method can be UV detected at 200 nm with very high resolution.
Column | Primesep 100, 3.2×100 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 30/70% |
Buffer | Gradient H2SO4 – 0.05- 0.8%, 10 min |
Flow Rate | 0.5 ml/min |
Detection | UV, 255 nm |
Class of Compounds |
Drug |
Analyzing Compounds | Acyclovir, Valacyclovir, Oseltamivir |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsOseltamivir
Valacyclovir
HPLC Analysis of Acyclovir
August 6, 2015
Acyclovir (also spelled aciclover) is an anti-viral drug on the World Health Organization list of essential medicines due to its use in the prevention and treatment of herpes simplex virus, varicella zoster virus and the shingles. Legacy L1 was used to retain aciclovir by reverse phase mechanism. Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.
Application Column
Legacy L1
SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.
Select options