Cefuroxime

Cefuroxime structural formula

CAS Number55268-75-2
Molecular FormulaC16H16N4O8S
Molecular Weight424.381
InChI KeyJFPVXVDWJQMJEE-QMTHXVAHSA-N
LogP-0.160
Synonyms
  • Cefuroxime
  • (6R,7R)-3-[(Carbamoyloxy)methyl]-7-[2-(furan-2-yl)(methoxyimino)acetamido]-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
  • 5-Thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 3-[[(aminocarbonyl)oxy]methyl]-7-[[2-(2-furanyl)-2-(methoxyimino)acetyl]amino]-8-oxo-, (6R,7R)-
  • 55268-75-2
  • 5-Thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 3-[[(aminocarbonyl)oxy]methyl]-7-[[(2Z)-2-furanyl(methoxyimino)acetyl]amino]-8-oxo-, (6R,7R)-
  • 5-Thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 3-[[(aminocarbonyl)oxy]methyl]-7-[[(2Z)-2-(2-furanyl)-2-(methoxyimino)acetyl]amino]-8-oxo-, (6R,7R)-
  • 5-Thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid, 3-[[(aminocarbonyl)oxy]methyl]-7-[[2-furanyl(methoxyimino)acetyl]amino]-8-oxo-, [6R-[6?,7?(Z)]]-
  • Biofuroksym
  • Cefaloxime
  • Cefasyn
  • Cefuroxim
  • Cefuroxime acid
  • cefuroximo
  • Cephuroxime
  • Ketocef
  • BRN 5783190
  • EINECS 259-560-1
  • Cefuril
  • Zinacef Danmark
  • Cefuroximum
  • UNII-O1R9FJ93ED

Applications:

HPLC Method for Analysis of Cefuroxime on Primesep 100 Column

January 27, 2023

HPLC Method for Analysis of Cefuroxime on Primesep 100 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of  Cefuroxime on Primesep 100 by SIELC Technologies
HPLC Method for Analysis of Cefuroxime on Primesep 100 Column by SIELC Technologies

Cefuroxime is a cephalosporin antibiotic used to treat a variety of infections including bronchitis, gonorrhea, and Lyme disease. This antibiotic can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a sulfuric acid (H2SO4) buffer. This analysis method can be detected in the UV regime at 190 and 275 nm.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cefuroxime

Condition

ColumnPrimesep 100, 4.6×150 mm, 5 µm, 100A
Mobile PhaseMeCN/H2O – 20/80%
BufferH2SO4 – 0.05%
Flow Rate1.0 ml/min
DetectionUV 190, 275 nm
Peak Retention Time4.81 min

Description

Class of CompoundsDrug
Analyzing CompoundsCefuroxime

Application Column

Primesep 100

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Cefuroxime
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of Cefuroxime on Newcrom R1 HPLC column

February 16, 2018
Separation of Cefuroxime on Newcrom C18 HPLC column

Cefuroxime can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Cefuroxime
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.