Basic Blue 47

Basic Blue 47 structural formula

CAS Number12217-43-5
Molecular FormulaC23H21N3O2
Molecular Weight371.440
InChI KeyIVFRHOQHKQWEHJ-UHFFFAOYSA-N
LogP3.78
Synonyms
  • Basic Blue 47
  • 1-Amino-4-{4-[(dimethylamino)methyl]anilino}anthracene-9,10-dione
  • 9,10-Anthracenedione, 1-amino-4-[[4-[(dimethylamino)methyl]phenyl]amino]-
  • 12217-43-5
  • EINECS 235-398-7
  • UNII-8G3UK6793U
  • 1-Amino-4-[[4-[(dimethylamino)methyl]phenyl]amino]anthraquinone

Applications:

Separation of Basic Blue 47 on Newcrom R1 HPLC column

May 16, 2018
Separation of Basic Blue 47 on Newcrom R1 HPLC column

Basic Blue 47 can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
Basic Blue 47
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.