Four underivatized amino acids (serine, methylserine, GABA and methionine) were separated on a Primesep 100 reversed-phase cation-exchange mixed-mode HPLC column. Two methods, one for UV and one for ELSD/LC/MS show good separation and peak shape for underivatized amino acids. This column and general HPLC approach can be used for analysis of underivatized amino acids. Primesep 100 is designed to replace reversed-phase HPLC column in combination with ion-pairing reagents.
| Column | Primesep 100, 4.6×250 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | H3PO4 or Formic acid |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 200 nm, ELSD |
| Class of Compounds | Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid |
| Analyzing Compounds | Serine, Methylserine, GABA, Methionine |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
MethionineSerine
a-Methylserine
gamma-Aminobutyric Acid (GABA)
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
