alpha-Arbutin

alpha-Arbutin structural formula

CAS Number84380-01-8
Molecular FormulaC12H16O7
Molecular Weight272.252
InChI KeyBJRNKVDFDLYUGJ-ZIQFBCGOSA-N
LogP-1.29
Synonyms
  • alpha-Arbutin
  • 4-Hydroxyphenyl alpha-D-glucopyranoside
  • alpha-D-Glucopyranoside, 4-hydroxyphenyl
  • 84380-01-8
  • UNII-72VUP07IT5

Applications:

UV-Vis Spectrum of α-Arbutin

July 15, 2024

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.

Application Analytes:
alpha-Arbutin
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

HPLC Method for Analysis of Alpha – Arbutin on Primesep N Column

February 16, 2024

HPLC MS Method for Analysis of alpha-Arbutin on Primesep N Column

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC Method for Analysis of alpha-Arbutin on Primesep N Column by SIELC Technologies


 High Performance Liquid Chromatography (HPLC) Method for Analysis of alpha-Arbutin

Alpha-arbutin is a naturally occurring compound found in certain plants, particularly in the bearberry (Arctostaphylos uva-ursi) plant, cranberries, blueberries, and pears. It is a glycoside derivative of hydroquinone, which means it has a glucose molecule attached to a hydroquinone molecule.

Skin Lightening: Alpha-arbutin is known for its skin-lightening properties. It is often used in skincare products to reduce the appearance of hyperpigmentation, such as dark spots and age spots.

Inhibition of Tyrosinase: Alpha-arbutin works by inhibiting the activity of the enzyme tyrosinase, which is involved in the production of melanin (the pigment responsible for skin color). By inhibiting tyrosinase, alpha-arbutin helps to reduce melanin production and, consequently, lighten the skin.

Cosmetic Products: Alpha-arbutin is commonly used as an ingredient in various skincare and cosmetic products, such as creams, serums, and lotions, aimed at promoting even skin tone and reducing the appearance of dark spots.

It’s essential to note that while alpha-arbutin is generally considered safe for topical use, individuals with sensitive skin or those undergoing specific skincare treatments should consult with a dermatologist or healthcare professional before using products containing this ingredient.

Alpha – Arbutin can be retained and analyzed using a Primesep N mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN). This method allows for detection using UV at 225 nm

ColumnPrimesep N, 4.6 x 150 mm, 5 µm, 100 A
Mobile PhaseMeCN 95%
BufferNone
Flow Rate0.2 ml/min
DetectionUV 225nm
Samples1 mg/ml
Injection volume1 µl
LOD*11 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Antiarrhythmic agent, Amide
Analyzing Compoundsalpha-Arbutin

Application Column

Primesep N

Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
alpha-Arbutin

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Separation of alpha-Arbutin on Newcrom R1 HPLC column

May 16, 2018
Separation of alpha-Arbutin on Newcrom R1 HPLC column

alpha-Arbutin can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.

Application Column

Newcrom R1

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Application Analytes:
alpha-Arbutin
The result was obtained by a proprietary SIELC algorithm. It may deviate from the actual experimental data. The experimental data are available upon request. Contact us by e-mail: support@sielc.com or by phone: 847-229-2629.