HPLC Separation of Agricultural Compounds Found in Tea

  Tea has been known to contain residue of pesticides and fungicides such as picoxystrobin and pyrimethanil. We used Primesep 200, a reversed-phase column with weakly acidic ion-pairing groups to separate picoxystrobin, pyrimethanil and atorvastatin. A 15 minute method used to demonstrate retention control when adjusting ratio of organic to aqueous in the mobile phase. Baseline retention with good resolution was achieved.    


Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 45/55%, MeCN/H2O - 50/50%
Buffer H2SO4 - 0.1 %
Flow Rate 1.0 ml/min
Detection UV, 250 nm


Class of Compounds Drug, Statin, Hydrophobic, Ionizable
Analyzing Compounds Atorvastatin, Picoxystrobin, Pyrimethanil, Lipitor
Application Analytes: