Tea has been known to contain residue of pesticides and fungicides such as picoxystrobin and pyrimethanil. We used Primesep 200, a reversed-phase column with weakly acidic ion-pairing groups to separate picoxystrobin, pyrimethanil and atorvastatin. A 15 minute method used to demonstrate retention control when adjusting ratio of organic to aqueous in the mobile phase. Baseline retention with good resolution was achieved.
| Column | Primesep 200, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 45/55%, MeCN/H2O – 50/50% |
| Buffer | H2SO4 – 0.1 % |
| Flow Rate | 1.0 ml/min |
| Detection | UV, 250 nm |
| Class of Compounds |
Drug, Statin, Hydrophobic, Ionizable |
| Analyzing Compounds | Atorvastatin, Picoxystrobin, Pyrimethanil, Lipitor |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsPicoxystrobin
Pyrimethanil
