HPLC Method for Analysis of MDMA


Methylenedioxymethamphetamine (MDMA), also known as ecstasy, is a synthetic drug that has both, a psychedelic and stimulant effects. It is predominantly a “club drug” and is commonly used at rave parties. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain MDMA. The method is UV compatible and can be used as a general approach for analyzing similar compounds.


Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%
Buffer Gradient H3PO4 - 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm


Class of Compounds Drug,  Acid, Hydrophilic, Ionizable,
Analyzing Compounds Methylenedioxymethamphetamine (MDMA)

Application Analytes: