HPLC Method for Analysis of Butorphanol

Chr_1048 Butorphanol is a synthetic morphinan analgesic. It has narcotic antagonist action and is used to manage severe pain and migraines as a supplement for balanced general anesthesia. Primesep 200, a reverse phase column, contains embedded acidic ionizable groups and can retain Butorphanol. The method is UV compatible and can be used as a general approach for analyzing similar compounds.


Column Primesep 200, 3.2x100 mm, 5 µm, 100A
Mobile Phase Gradient MeCN - 5-50%
Buffer Gradient H3PO4 - 0.05-0.3%, 5 min, 4 min hold
Flow Rate 0.5 ml/min
Detection UV, 270 nm


Class of Compounds Drug,  Acid, Hydrophilic, Ionizable,
Analyzing Compounds Butorphanol

Application Analytes: