Applications:
Separation of Sulphonium Ions in Polar Organic Mode
October 4, 2005
The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O – 90/10% |
Buffer | TFA – 0.1% |
Flow Rate | 1.5 ml/min |
Detection | ELSD |
Class of Compounds |
Acid, Hydrophilic, Ionizable |
Analyzing Compounds | Sulphonium Ions, Methoxydimethylsulphonium, Trimethylsulfphonium |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Methoxydimethylsulphonium
Sulfonium Ions
Trimethylsulfonium