Glycine Betaine

Glycine Betaine

CAS Number107-43-7
Molecular FormulaC5H11NO2
Molecular Weight117.148
  • Betaine
  • (Trimethylazaniumyl)acetate
  • Methanaminium, 1-carboxy-N,N,N-trimethyl-, inner salt
  • 107-43-7
  • 4-04-00-02369
  • Methanaminium, 1-carboxy-N,N,N-trimethyl-, inner salt
  • (Carboxymethyl)trimethylammonium hydroxide inner salt
  • (Trimethylammonio)acetate
  • 2-(Trimethylammonio)ethanoic acid, hydroxide, inner salt
  • Abromine
  • Aminocoat
  • Ammonium compounds, substituted, (carboxymethyl)trimethyl-, hydroxide, inner salt
  • Aquadew AN 100
  • Betafin
  • Betafin BCR
  • Betafin BP
  • Betafin BP 20
  • betaina
  • Cystadane
  • Fencaijian
  • FinnStim
  • Glycine betaine
  • Glycine, trimethylbetaine
  • Glycocoll betaine
  • Glycylbetaine
  • Greenstim
  • Loramine AMB 13
  • Methanaminium, 1-carboxy-N,N,N-trimethyl-, hydroxide, inner salt
  • N,N,N-Trimethylglycine
  • Oxyneurine
  • Rubrine C
  • Trimethylbetaine
  • Trimethylglycine
  • Trimethylglycocoll
  • α-Earleine
  • BRN 3537113
  • (Carboxymethyl)trimethylammonium hydroxide, inner salt
  • EINECS 203-490-6
  • Jortaine
  • NSC 166511
  • Glykokollbetain
  • UNII-3SCV180C9W
  • Acidin-Pepsin
  • Novobetaine
  • Hepastyl
  • (trimethylammoniumyl)acetate
  • 1-Carboxy-N,N,N-trimethyl-Methanaminium
  • 1-Carboxy-N,N,N-trimethyl-Methanaminium hydroxide
  • 1-carboxy-N,N,N-trimethylmethanaminium inner salt
  • 2-(trimethylazaniumyl)acetate
  • 2-N,N,N-trimethylammonio acetate
  • 2-trimethylammonioacetate
  • 2-trimethylammonioethanoate
  • Bet
  • Ektasolve EE
  • Loramine AMB-13
  • Lycine
  • N,N,N-trimethylammonioacetate
  • Trimethylaminoacetic acid
  • Trimethylammonioacetate
  • Trimethylbetaine Glycine
  • a-Earleine
  • acidol
  • alpha-Earleine
  • 11042-12-9
  • 24980-93-6
  • 45631-77-4
  • 590-30-7


HPLC Analysis of Cheese Extract using Mixed-Mode Chromatography


Primesep 100 separates the components of a Swiss cheese extract by HPLC using cation exchange and reversed phase as retention mechanisms. The amino acids, glutamic acid and proline, as well as glycine betaine were resolved in less than 10 minutes. A mobile phase gradient of water, acetonitrile (MeCN, ACN,) and phosphoric acid (H3PO4) and ultraviolet (UV) detection was used.


Column Primesep 100, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV 210 nm



Class of Compounds
Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Glutamic acid,  Proline, Glycine betaine


Application Column

Primesep 100

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Cheese Extract
Glutamic Acid
Glycine Betaine

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to so we can ensure you get optimal results for your compound/s of interest.