USP Methods for the Analysis of Aminocaproic Acid using the Legacy L1 Column

  Application Notes: Aminocaproic acid is a enzymatic inhibitor. According to USP methods, aminocaproic acid conatins not less than 98.5% and not more than 101.5% of aminocaproic acid calculated on a dried basis. The USP HPLC method for the separation of aminocaproic acid was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.   Application Columns: Legacy L1 C18 HPLC column Application compounds: Aminocaproic acid Mobile phase: Sodium heptanesulfonate/H2O/MeOH /NaH2PO4 pH 2.2 Detection technique: UV Reference: USP35: NF30


Column Legacy L1, 4.6x150 mm, 5 µm, 100A
Mobile Phase Sodium heptanesulfonate/H2O/MeOH /NaH2PO4 pH 2.2
Flow Rate 0.7 ml/min
Detection UV, 210 nm


Class of Compounds Drug, Clotting promoter, Hydrophilic, Ionizable
Analyzing Compounds Aminocaproic Acid
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Application Analytes:
Aminocaproic acid

Application Detection:
UV Detection