The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both polar organic and ion-exchange modes in the separation of sulfonium ions. The high organic mobile phase retains methane sulfonic acid by a polar organic mechanism and separates methoxydimethylsulphonium and trimethylsulfphonium cations by polar organic and cation exchange modes. The separation method uses a mobile phase mixture of water, acetonitrile (MeCN, ACN), and trifluoroacetic acid (TFA) with evaporative light scattering detection (ELSD).
| Column | Primesep 100, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 90/10% |
| Buffer | TFA – 0.1% |
| Flow Rate | 1.5 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Sulphonium Ions, Methoxydimethylsulphonium, Trimethylsulfphonium |
Application Column
Primesep 100
Column Diameter: 4.6 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Application Analytes:
Methanesulfonic AcidMethoxydimethylsulphonium
Sulfonium Ions
Trimethylsulfonium
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
