The separation of steroids on Primesep 100 demonstrates the versatility of Primesep columns in both reversed- and normal-phase modes. Switching modes by changing the mobile phase reverses peaks 2 and 3. The separation method uses a mobile phase mixture of water and acetonitrile (MeCN, ACN) for reversed phase and hexane and ethanol for normal phase with UV detection at 240 nm.
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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Primesep C
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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