Dextromethorphan is used as a temporary cough suppressant in many medications. The elution time of dextromethorphan and bromide can be reversed in HPLC chromatography on a Primesep B2 reverse-phase (RP) column with embedded basic ion-pairing groups by only changing the concentration of buffer in a simple mobile phase of water and acetonitrile (MeCN, ACN). Buffer is trifluoroacetic acid (TFA). UV detection at 210nm.
Application Column
Primesep B2
Column Diameter: 3.2 mm
Column Length: 150 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
Dextromethorphan