Retention of Sialic Acid on Primesep D Column

Sialic acid is a nitrogen or oxygen substituted neuraminic acid. It is a sugar-based derivative with very polar properties. The N-substituted compound is acidic in nature. Sialic acid has no retention in reversed-phase, unless an ion-pairing reagent is used. The method for analysis of sialic acid was developed on the Primesep D reversed-phase anion-exchange column. The method is compatible with ELSD and LC/MS and also can be used for the analysis of sialic acid in biological fluids like blood, serum, urine, etc. Retention time of sialic acid is controlled by the pH of the mobile phase and the buffer concentration. It is retained by very weak, reversed-phase and weak, anion-exchange mechanisms.  


Column Primesep D, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements
Analyzing Compounds Sialic Acid
Application Analytes:
Sialic Acid