Primesep 200 separates the very hydrophilic gentamicin isomers. Gentamicin contains multiple primary and secondary amines as well as multiple hydroxyl groups. Ion-exchange and other polar interactions provide retention for a compound that elutes in the void of a traditional C8 column. The separation uses a simple mobile phase of water, acetonitrile (MeCN, ACN), and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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