HPLC Separation of Gentamicins

Primesep 200 separates the very hydrophilic gentamicin isomers. Gentamicin contains multiple primary and secondary amines as well as multiple hydroxyl groups. Ion-exchange and other polar interactions provide retention for a compound that elutes in the void of a traditional C8 column. The separation uses a simple mobile phase of water, acetonitrile (MeCN, ACN), and trifluoracetic acid (TFA) with evaporative light scattering detection (ELSD).

Application Column

Primesep 200

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

Select options
Application Analytes:
Gentamicin Sulfate

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.