HPLC Separation of Epinephrine and Related Impurities

Epinephrine (also referred to as adrenaline) is a hormone and neurotransmitter. It is a catecholamine, a sympathomimetic monoamine derived from the amino acids phenylalanine and tyrosine. Epinephrine is polar basic compounds and it is retained on mixed-mode cation exchange columns without ion-pairing reagent. Epinephrine is retained by weak reversed phase and strong cation-exchange mechanisms. Formulations for epinephrine might have citric and ascorbic acid and EDTA. These three compounds are not retained by cation-exchange or reversed-phase mechanisms and elute in the void. Current HPLC method can be used for quantitation of epinephrine and recepinephrine (recemate) in various compositions. Epinephrine and related impurity can be monitored by UV, ELCD/MS, ELSD or Corona CAD. Corresponding buffer is required for each detection technique.


Column Primesep 200,  4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer NaH2PO4
Flow Rate 1.0 ml/min
Detection 410


Class of Compounds Ions,  Hydrophilic, Ionizable
Analyzing Compounds Ascorbic acid, Citric acid, EDTA, Racepinephrine

Application Analytes: