HPLC Separation of Citrulline and Arginine

Citrulline and Arginine are α-amino acids that differ only in one functional group. They can be separated on a reverse-phase Primesep 200 HPLC column by hydrophobic and cation-exchange mechanisms in a mobile phase of water and acetonitrile (MeCN, ACN) with a gradient buffer of formic acid. Evaporative Light Scattering Detector (ELSD) used.


Column Primesep 200,  4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer Formic Acid
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Ions,  Hydrophilic, Ionizable
Analyzing Compounds Sodium, Citrulline, Arginine
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Application Analytes:

Application Detection:
ELSD/MS Detection