HPLC Separation of Chlorate and Bromate Ions

Primesep B2 separates chlorate and bromate by an ion-exchange mechanism. The anion-exchange properties of the column allow retention of hydrophilic compounds, and these properties can be adjusted by simply changing the ammonium formate buffer concentration. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN) and ammonium formate with evaporative light scattering detection (ELSD).

Condition
Column Primesep B2, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer AmFm pH 3.0
Flow Rate 1.0 ml/min
Detection CAD

Description
Class of Compounds Ions, Hydrophilic, Ionizable
Analyzing Compounds Sodium Chlorate, Sodium Bromate

Application Column

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Bromate
Chlorate
Sodium Bromate
Sodium Chlorate
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.