HPLC Separation of Antibiotics in Fish Production

Five antibiotics are separated on Primesep C mixed-mode cation-exchange column using UV, ESLD and LC/MS compatible conditions. Similar compounds can be separated based on reverse phase and cation-exchange mechanisms. Current method can be used in quantitative determination of antibiotics in various food products.

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep C

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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Application Analytes:
5-Nitro-2-Furaldehyde Semicarbazone (Nitrofurazone)
Chloramphenicol
Furaltadone
Furazolidone
Nitrofurantoin

Application Detection:
UV Detection