HPLC Separation of Amino Acids

Amino acids are building blocks for peptides and proteins. Serine is not essential to human diet and it is synthesized in human body. Methionine is not synthesized in human body and needs to be ingested. GABA is used to enhance growth of specified plants, prevent development of powdery mildew on grapes, and suppress certain other plant diseases. In humans GABA helps to maintain normal brain function. Serine, methylserine, GABA and methionine are separated on Primesep 100 column by mixed-mode mechanism. Amino acids are retained by combination of reverse phase and cation-exchange mechanisms. At lower pH carboxylic acid fragment of amino acid is suppressed and not ionized, making amino acids more basic and slightly more hydrophobic. Amount of acetonitrile, buffer concentration and buffer pH can be used to adjust retention time. Underivatized amino acids are well retained and separated with perfect peak shape and symmetry. Fast method can be used for UV, ESLD and LC/MS quantitation of serine, methylserine, GABA and methionine.


Column Primesep 100, 4.6x250 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer H3PO4
Flow Rate 1.0 ml/min
Detection UV, 200 nm, ELSD


Class of Compounds Drug, Acid, Hydrophilic, Ionizable, Vitamin, Supplements, Amino acid
Analyzing Compounds Serine, Methylserine, GABA, Methionine
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Application Analytes:
gamma-Aminobutyric Acid (GABA)

Application Detection:
UV Detection
ELSD/MS Detection