HPLC Separation of Alkyl Sulfonic Acids

Alkyl sulphonic acids are used as surfactant additives in many products. Homologs of linear alkyl sulfonates are separated on a Primesep 500 mixed-mode cation exchange column in reversed-phase anion-exclusion mode. This generic method can be used for determination of alkyl sulfonates in detergents, soaps, etc.


Column Primesep 500,  4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O
Buffer AmAc
Flow Rate 1.0 ml/min
Detection ELSD



Class of Compounds
Acid,  Hydrophilic, Ionizable
Analyzing Compounds Sodium, Hexanesulphonic Acid, Heptanesulphonic Acid, Octanesulphonic Acid

Application Column

Primesep 500

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Heptanesulfonic Acid
Hexanesulfonic Acid
Octanesulfonic Acid

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.