HPLC Separation of Alendronic, Ibandronic, Etidronic Acids

Dronic acids are bisphosphonates that differ in their alkyl chains. Alendronate (Fosamax) marketed alone is used for osteoporosis and other bone diseases. It is also combined with Vitamin C (Fosavance). A short, 50 mm Primesep SB column separates alendronic, ibandronic, and etidronic acids. The HPLC separation uses a mobile phase of water, acetonitrile (MeCN, ACN), trifluoracetic acid (TFA) or ammonium formate and evaporative light scattering detection (ELSD) at 80°C.

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Alendronic Acid
Etidronic Acid
Ibandronic Acid
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.