HPLC Separation of 1-Methylhistidine and 3-Methylhistidine on Primesep 200

  Application Notes: Methylhistidines are polar amino acids with very similar structures. Methylhistidines are methylated derivative of histidine found in actin. N-methylhistidine is released into urine after the breakdown of actin and myosin. Urinary output of N-methylhistidine is a reliable index of the rate of myofibrillar protein breakdown in musculature. The method for separation of 1-methylhistidine and 3-methylhistidine on Primesep 200 column was developed with LC/MS compatible conditions. This method can be used for quantitation of methylhystidines in urine and other biofluids. Application Columns: Primesep 200 Application compounds: 1-Methylhistidine, 3-Methylhistidine Detection technique: UV, LC/MS, ELSD/CAD  


Column Primesep 200, 4.6x150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O - 20/80%
Buffer AmFm pH 3.0- 20 mM
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds Amino acid , Acid, Hydrophilic, Ionizable
Analyzing Compounds 1-Methylhistidine, 3-Methylhistidine
Application Analytes: