HPLC Retention of Sulfamic Acid on Primesep SB Mixed-Mode Column

Sulfamic acid is inorganic acid which is used in various synthesis and compositions. It is used as precursor in synthesis of artificial sweeteners. Sulfamic acid (amidosulfonic acid, amidosulfuric acid, aminosulfonic acid, and sulfamidic acid) is very hydrophilic in nature and does not retain on reversed-phase columns. Acidic nature of this acid allows to retain this compound by mixed-mode mechanism. In current method sulfamic acid is retained by anion-exchange mechanism on Primesep SB mixed-mode anion-exchange column. Method employes acetonitrile and ammonium formate or formic acid. Sulfamic acid has no UV activity and needs to be detected by LC/MS, ELSD or Corona CAD. Retention time of sulfamic acid can be adjusted by the amount of buffer/acid in the mobile phase. This method can be used for analysis of organic and inorganic acids in different sample matrices.

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Amidosulfonic Acid
Aminosulfonic Acid
Sulfamic Acid
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.