HPLC Retention of Obidoxime


Obidoxime is a highly polar compound containing two quaternary amines linked by short hydrophilic chain. It is not retained well in reverse phase chromatography and produces severe tailing due to interaction with residual silanol groups of reverse phase HPLC columns. Obidoxime is retained on Primesep C column by strong cation-exchange and weak reverse phase mechanisms. Primesep C column has carboxylic group on the silica surface which shields residual silanols from interacting with quaternary amine, thus providing improved peak shape for quaternary amine. Retention time of hydrophilic quaternary amine can be adjusted by pH and buffer concentration. This reliable and fast method can be used to retain and quantitate obidoxime and other polar hydrophilic quaternary amine on Primesep C column. Different buffers and detection techniques can be used to monitor purity of obidoxime. Obidoxime is a member of the oxime family used to treat nerve gas poisoning. Oximes are drugs known for their ability to reverse the binding of organophosphorus compounds to the enzyme acetyl cholinesterase.

Application Column

Primesep C

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:

Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.