HPLC Retention of N-Cyclohexylsulfamic Acid


N-Cyclohexylsulfamic acid (cyclamic acid), sodium or calcium salts artificial sweeteners. Cyclamate is hydrophilic strongly acidic compound. Cyclamic acid was analyzed on a Primesep D HPLC column using acetonitrile water ammonium formate buffer and ELSD. This molecule is not UV active and can be monitored by ELSD, Corona, or LC/MS detection. Compound is retained by weak reversed-phase and strong anion-exchange mechanism.

Application Column

Primesep D

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
N-Cyclohexylsulfamic Acid
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.