HPLC Analysis of Valproic Acid using Mixed-Mode Chromatography

Separation type: Liquid Chromatography Mixed-mode








Valproic acid is a chemical compound used as a convulsant and mood-stabilizing drug. Valproic acid is hydrophobic compound containing carboxylic acid. It is retained on a Primesep B2 column by combination of reversed-phase and anion-exchange mechanism.



Condition
Column Primesep B2, 4.6×50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer Ammonum Acetate pH 4.0 – 10 mM
Flow Rate 1.0 ml/min
Detection ELSD

 

Description
Class of Compounds
  Acid
Analyzing Compounds Valproic Acid

 

Application Column

Primesep B2

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Valproic Acid
Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.