HPLC Analysis of Valproic Acid using Mixed-Mode Chromatography

Separation type: Liquid Chromatography Mixed-mode

Valproic acid is a chemical compound used as a convulsant and mood-stabilizing drug. Valproic acid is hydrophobic compound containing carboxylic acid. It is retained on a Primesep B2 column by combination of reversed-phase and anion-exchange mechanism.


Column Primesep B2, 4.6x50 mm, 5 µm, 100A
Mobile Phase MeCN
Buffer Ammonum Acetate pH 4.0 - 10 mM
Flow Rate 1.0 ml/min
Detection ELSD


Class of Compounds  Acid
Analyzing Compounds Valproic Acid
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Application Analytes:
Valproic Acid

Application Detection:
ELSD/MS Detection