HPLC Analysis of L-Cysteine-S-Sulfate on Primesep SB Column

Cysteine-S-sulfate (SSC) is produced by reaction of inorganic sulfite and cystine by a as-yet-unknown pathway, and is a very potent NMDA-receptor antagonist. Cysteine-S-sulfate is a very polar molecule with one basic group, one carboxylic group and one sulfate group. It is retained by Primesep SB column by combination of strong anion-exchange mechanism and weak reversed-phase mechanism. Compound can be monitored by LC/MS, ELSD, Corona or UV detectors. This HPLC method can be adopted as generic approach for analysis of acidic and hydrophobic drugs.

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Primesep SB

The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.

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ELSD/MS Detection