Diphenamid is an herbicide which selectively controls weedy grasses and broadleaf plants in the production of tobacco, tomatoes, peppers, cotton, and many more commercial crops. Diphenamid is a preemergence herbicide that is surface applied to soils and can be expected to control weeds for 6-8 months. Diphenamid was analyzed on two different stationary phases. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.
Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 25-70% |
Buffer | Gradient AmAc pH 3.0- 30-60 mM |
Flow Rate | 0.4 ml/min |
Detection | UV, 270 nm |
Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 10-70% |
Buffer | Gradient AmAc pH 3.0- 20-60 mM |
Flow Rate | 0.4 ml/min |
Detection | UV, 270 nm |
Column | Primesep 100, 2.1×150 mm, 5 µm, 100A |
Mobile Phase | Gradient MeCN – 10-70% |
Buffer | Gradient AmAc pH 3.0- 20-60 mM |
Flow Rate | 0.4 ml/min |
Detection | UV, 270 nm |
Class of Compounds |
Herbicide, Insecticide, Hydrophobic, Ionizable |
Analyzing Compounds | Diphenamid |
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select optionsThe Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
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