Diphenamid is an herbicide which selectively controls weedy grasses and broadleaf plants in the production of tobacco, tomatoes, peppers, cotton, and many more commercial crops. Diphenamid is a preemergence herbicide that is surface applied to soils and can be expected to control weeds for 6-8 months. Diphenamid was analyzed on two different stationary phases. Primesep 100 is a reverse phase column that contains embedded acidic ion-pairing groups, while Obelisc R retains with long hydrophobic chains and multiple ionic pairing groups on the surface. Method is LC/MS compatible and can be replicated to analyze dozens of other pesticides.
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 25-70% |
| Buffer | Gradient AmAc pH 3.0- 30-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70% |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Primesep 100, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70% |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Herbicide, Insecticide, Hydrophobic, Ionizable |
| Analyzing Compounds | Diphenamid |
Application Analytes:
DiphenamidApplication Detection:
UV Detection
Obelisc R
SIELC has developed the mixed-mode Obelisc™ columns to be the first commercially available columns with Liquid Separation Cell technology (LiSC™). This cost effective duo can replace multiple HPLC columns such as reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion- exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - Buffer concentration, Buffer pH, and Organic Modifier Concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
Select options
Primesep 100
The Primesep family of mixed-mode columns offer a wide variety of stationary phases with an unprecedented selectivity in the separation of a broad array of chemical compounds and in multiple applications. Corresponding Primesep guard columns are available with all stationary phases and do not require holders. SIELC offers a method development service which is available for all customers. Ask about our special custom LC-phases tailored for specific separations.
Select options
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.
