HILIC Separation of Hydroxypiperidine and Impurities

Primesep 200 mixed-mode column is used in HILIC/cation-exchange mode to separate hydroxypiperidine and related impurities. Compounds are monitored by ELSD in the presence of trifluoroacetic acid in the mobile phase.

Application Column

Primesep 200

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options

Hydroxypiperidine

ELSD Detection

SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.

Alltesta™ Cromite Analyzer

The Cromite™ Analyzer represents a new generation of affordable and easy-to-learn HPLC instrumentation for schools, colleges and universities.

With automated single-vial injection, high-pressure flow and UV/Vis detection capabilities, scholars can use this instrument to separate compounds, such as vitamins in juices, food dyes, caffeine and active ingredients in pharmaceutical products. The cloud-connected software allows you to run tests, analyze results and share data from anywhere and with any device. SIELC Technologies provides a vast library of free methods for a variety of compounds, enabling students to characterize and understand the chemical composition of many different products. The Cromite™ Analyzer comes with a column of your choice and all necessary accessories to perform multiple analyses. For pricing information, please contact us at

Alltesta™ Gradient Automated Analyzer

SIELC's Alltesta™ Analyzer is an affordable HPLC analytical system that provides both scientific and educational environments with a convenient, simple solution for measuring small and trace concentrations of various chemicals in products such as milk, baby formula, pet food, pills, household formulations, and more. For pricing information, please contact us at

Alltesta™ Mini Autosampler

Based on a proprietary alpha/beta mechanic configuration, this miniature autosampler offers inexpensive and reliable automation for many analytical- and liquid-handling settings, including HPLC. Direct and comprehensive control of this small autosampler’s features, as well as high-level commands for succinct automation, provide quick and easy integration. Users can customize their mini autosampler with various valve configurations, tray designs, and syringe volumes and pressures. A built-in shaking feature allows for sample-mixing before injection and/or simple sample extraction inside the vial. The price of the Alltesta™ Mini Autosampler varies depending on the specific configuration requested. For more information on pricing, please contact us at