Separation of Nucleotide Monophosphates

 

Nucleotides are the monomers of DNA and RNA, composed of a five-carbon sugar, a nitrogenous base, and at least one phosphate group. Primesep SB is a good column for separating these highly polar compounds. Primesep SB is a reverse-phase column with strong embedded basic ion-pairing groups. Retention can me manipulated by adjusting acetonitrile, and the baseline separation can be achieved in under 6 minutes using a gradient. 

Application Column

Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

Select options
Application Analytes:
Adenosine Monophosphate
Cytidine Monophosphate
Guanosine Monophosphate
Inosine Monophosphate
Uridine Monophosphate
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.