Lactic acid (milk acid) is a hydrophilic acid which plays important role in many bioprocesses. Lactic acid has a tendency to self-condense to form dimers, trimers and high molecular weight oligomers. Lactic acid and it’s oligomers are retained by a combination of weak reversed-phase and weak anion-exchange mechanisms on Primesep D mixed-mode HPLC column. Retention time is controlled by amount of acetonitrile, buffer concentration and buffer pH. Method is compatible with LC/MS and other detection techniques and can be used for analysis of lactic acid in various matrices.
| Column | Primesep D, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 30/70% |
| Buffer | AmAc pH 4.0- 20 mM |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Acid, Hydrophilic, Ionizable |
| Analyzing Compounds | Lactic Acid |
Application Column
Primesep D
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsLactic Acid Oligomers
UV Detection
