Spermidine

HPLC Method for Analysis of Spermidine on Obelisc R by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Spermidine

Spermidine is a polyamine compound that is found in all living cells. It is a biogenic amine and is involved in various cellular processes.

Biological Roles:

• Cell Growth and Proliferation: Spermidine is involved in the regulation of cell growth and proliferation.
• DNA Stability: It interacts with DNA and RNA, contributing to their stability.
• Antioxidant Properties: Spermidine has been suggested to have antioxidant properties, potentially protecting cells from oxidative stress.
• Autophagy Induction: Spermidine has been shown to induce autophagy, a cellular process that involves the degradation of cellular components.

Natural Sources:

• Spermidine is found in various foods, including wheat germ, soybeans, mushrooms, and aged cheese.
• It is also synthesized within the body from other polyamines.

Research and Health Implications:

• Research on spermidine has gained attention for its potential health benefits, including its role in promoting longevity and protecting against age-related diseases.
• Some studies suggest that spermidine supplementation may have positive effects on cardiovascular health, neuroprotection, and other aspects of aging.

Supplementation:

• Spermidine is available as a dietary supplement, and some people choose to take it for its potential health benefits.
• However, it’s important to note that while research is ongoing, the long-term effects and optimal dosage for supplementation are not fully established.

Spermidine can be retained and analyzed using an Obelisc R mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD