| CAS Number | 59-56-3 |
|---|---|
| Molecular Formula | C6H13O9P |
| Molecular Weight | 230.14 |
| InChI Key | HXXFSFRBOHSIMQ-VFUOTHLCSA-N |
| LogP | -3.8 |
| Synonyms |
|
Applications:
HPLC Separation of Glucose 6- Phosphate and Glucose 1- Phosphate on Newcrom B Column
January 20, 2020
Separation type: Liquid Chromatography Mixed-mode
Structural isomer separation poses a challenging task when using reverse-phase chromatography. However both glucose
variants, which only differ to which carbon the phosphate group is attached to, can be separated in HPLC isocratically on a
reverse-phase mixed-mode anion-exchange Newcrom B column.
What differentiates, the Newcrom B phase from other silica-based
Analysis of glucose phosphate is important because glucose-6-phosphate is the product of the first step in glycolysis
metabolic pathway and glucose-1-phosphate is the first step in glycogenolysis. Glucose-6-phosphate is converted to
glucose-1-phosphate and vice versa by the phosphoglucomutase enzyme in biological systems.
High Performance Liquid Chromatography (HPLC) Method for Analysis of Glucose 6- Phosphate and Glucose 1- Phosphate
| Column | Newcrom B, 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic Acid – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD, 40 °C MS- compatible mobile phase |
| Class of Compounds | Hydrophilic, Sugar |
| Analyzing Compounds | Glucose 6- Phosphate, Glucose 1- Phosphate |
Application Column
Newcrom B
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select optionsGlucose 6-Phosphate
