| CAS Number | 94-82-6 |
|---|---|
| Molecular Formula | C10H10Cl2O3 |
| Molecular Weight | 249.090 |
| InChI Key | YIVXMZJTEQBPQO-UHFFFAOYSA-N |
| LogP | 3.53 |
| Synonyms |
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Applications:
Controlling Retention of 2,4-DB on Mixed-Mode HPLC Columns
September 9, 2015
4-(2,4-dichlorophenoxy)butyric acid or 2,4-DB is a herbicides used in alfalfa, peanuts, and soybean production. 2,4-DB selectively controls broadleaf weeds inhibiting growth at the root and stem tips. It is considered slightly toxic, as is its metabolite 2,4-D. The EURL (European Union Reference Laboratory) included 2,4-DB in an analysis of acidic pesticides using QuEChERS methodology. Primesep 100 and Obelisc R were used to retain and separate 2,4-DB from impurities. Method is LC/MS compatible and developed to be used for many pesticides.
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 25-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 30-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Obelisc R, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Column | Primesep 100, 2.1×150 mm, 5 µm, 100A |
| Mobile Phase | Gradient MeCN – 10-70%, 15 min |
| Buffer | Gradient AmAc pH 3.0- 20-60 mM, 15 min |
| Flow Rate | 0.4 ml/min |
| Detection | UV, 270 nm |
| Class of Compounds |
Insecticide, Herbicide, Fungicide, Hydrophobic, Ionizable |
| Analyzing Compounds | 2,4-DB |
Application Column
Obelisc R
SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.
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Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
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