| CAS Number | 372-75-8 |
|---|---|
| Molecular Formula | C6H13N3O3 |
| Molecular Weight | 175.188 |
| InChI Key | RHGKLRLOHDJJDR-BYPYZUCNSA-N |
| LogP | -3.13 |
| Synonyms |
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Applications:
HPLC Analysis of Citrulline and Arginine on Primesep Columns
October 14, 2010
Two underivatized amino acids, citrulline and arginine, are separated by mixed-mode chromatography on Primesep 100 and Primesep 200 HPLC columns with LC/MS compatible mobile phase. Method can be used for retention and separation of underivatized amino acids without ion-pairing reagent in the mobile phase.
| Column | Primesep, 2.1×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | AmFm pH 3.0 |
| Flow Rate | 0.2 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Citrulline, Arginine, Chloride |
Application Column
Primesep 100
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCitrulline
HPLC Separation of Citrulline and Arginine on Primesep 200 Column
November 21, 2006
Citrulline and Arginine are α-amino acids that differ only in one functional group. They can be separated on a reverse-phase Primesep 200 HPLC column by hydrophobic and cation-exchange mechanisms in a mobile phase of water and acetonitrile (MeCN, ACN) with a gradient buffer of formic acid. Evaporative Light Scattering Detector (ELSD) used.
| Column | Primesep 200, 4.6×50 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O |
| Buffer | Formic Acid |
| Flow Rate | 1.0 ml/min |
| Detection | ELSD |
| Class of Compounds |
Ions, Hydrophilic, Ionizable |
| Analyzing Compounds | Sodium, Citrulline, Arginine |
Application Column
Primesep 200
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsCitrulline
Sodium
