USP Methods for the Analysis of Norepinephrine using the Legacy L1 Column

  Application Notes: Norepinephrine is a naturally occurring hormone and neurotransmitter. The USP HPLC method for the separation of norepinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.   Application Columns: Legacy L1 C18 HPLC column Application compounds: Norepinephrine Mobile phase: Sodium heptanesulfonate/H2O/MeOH 0.22/160/40  pH 3.0 Detection technique: UV Reference: USP35: NF30


Column Legacy L1, 4.6x250 mm, 5 µm, 100A
Mobile Phase Sodium heptanesulfonate/H2O/MeOH   0.22/160/40  pH 3.0
Buffer Sodium heptanesulfonate
Flow Rate 1.0 ml/min
Detection UV, 280 nm


Class of Compounds Drug, Hormone and neurotransmitter, Hydrophilic, Ionizable
Analyzing Compounds Norepinephrine
Application Analytes: