USP Method for the Analysis of Norepinephrine using the Legacy L1 Column


Application Notes: Norepinephrine is a naturally occurring hormone and neurotransmitter. The USP HPLC method for the separation of norepinephrine was developed on Legacy L1 column according to the US Pharmacopeia methodology. L1 classification is assigned to reversed-phase HPLC column containing C18 ligand. Support for the material is spherical silica gel with particles size 3-10 um and pore size of 100-120A. Resolution between critical pairs corresponds to rules and specifications of UPS.  

Application Columns: Legacy L1 C18 HPLC column

Application compounds: Norepinephrine

Mobile phase: Sodium heptanesulfonate/H2O/MeOH 0.22/160/40  pH 3.0

Detection technique: UV

Reference: USP35: NF30


Column Legacy L1, 4.6×250 mm, 5 µm, 100A
Mobile Phase Sodium heptanesulfonate/H2O/MeOH   0.22/160/40  pH 3.0
Buffer Sodium heptanesulfonate
Flow Rate 1.0 ml/min
Detection UV, 280 nm



Class of Compounds
Drug, Hormone and neurotransmitter, Hydrophilic, Ionizable
Analyzing Compounds Norepinephrine


Application Column

SIELC Legacy L1 HPLC column

Legacy L1

Column Diameter: 4.6 mm
Column Length: 250 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
UV Detection
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