Access the UV-Vis Spectrum SIELC Library

If you are looking for optimized HPLC method to analyze Quinoline Yellow WS check our HPLC Applications library

For optimal results in HPLC analysis, it is recommended to measure absorbance at a wavelength that matches the absorption maximum of the compound(s) being analyzed. The UV spectrum shown can assist in selecting an appropriate wavelength for your analysis. Please note that certain mobile phases and buffers may block wavelengths below 230 nm, rendering absorbance measurement at these wavelengths ineffective. If detection below 230 nm is required, it is recommended to use acetonitrile and water as low UV-transparent mobile phases, with phosphoric acid and its salts, sulfuric acid, and TFA as buffers.
For some compounds, the UV-Vis Spectrum is affected by the pH of the mobile phase.  The compound was dissolved with water. This analysis was done with the mobile phase made of 20% acetonitrile and 80% water.

HPLC Method for Analysis of Quinoline Yellow WS on BIST A+ Column by SIELC Technologies

Quinoline Yellow WS (E104, D&C Yellow 10) is a mixture of 3 different derivatives of Quinoline Yellow SS, consisting of monosulfonates, disulfonates, and trisulfonates. The dye is typically neon-yellow (yellow with a hint of green). It has the chemical formula C₁₈H₁₃NO_5/8/11S_1/2/3Na_1/2/3. The “WS” in it’s title stands in for “water-soluble.” It is used in foods, decorations, and coatings.

Using SIELC’s newly introduced BIST™ method, Quinoline Yellow WS can be retained and separated into its component compounds easily on a negatively-charged, cation-exchange BIST A+ column. There are two keys to this retention method: 1) a multi-charged, positive buffer, such as N,N,N’,N’-Tetramethyl-1,3-propanediamine (TMDAP), which acts as a bridge, linking the negatively-charged anion analytes to the negatively-charged column surface and 2) a mobile phase consisting mostly of organic solvent (such as MeCN) to minimize the formation of a solvation layer around the charged analytes. Other positively-charged buffers that can generate BIST™ include DMP, Calcium acetate, and Magnesium acetate. Using this new and unique analysis method, [comounds] can be retained and separated with high selectivity and great peak shape. This method can be detected and is compatible with ELSD, CAD, and Mass Spectrometry (LC-MS).

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