CAS Number | 58-56-0 |
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Molecular Formula | C8H12ClNO3 |
Molecular Weight | 205.640 |
InChI Key | ZUFQODAHGAHPFQ-UHFFFAOYSA-N |
LogP | -0.687 |
Synonyms |
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Applications:
HPLC Separation of Pyridoxine Hydrochloride (Vitamin B6) and Benfotiamine in Milgamma 100 Tablets on Primesep S Column
December 10, 2020
Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyridoxine Hydrochloride (Vitamin B6) and Benfotiamine
Pyridoxine Hydrochloride (also known as Vitamin B6), is a naturally occurring vitamin that can be found in a wide variety of common foods, such as meat, grains, and avocados. Benfotiamine is a synthetic form of Vitamin B1 that has shown early signs of treating nerve damage, Alzheimer’s, alcohol dependence. They can both be found in Milgamma 100 tablets, a medicine prescribed to treat Vitamin B1 and B6 deficiency.
These two vitamins can be detected in the low UV regime. Using a Primesep S normal-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid (H3PO4) buffer, Pyridoxine Hydrochloride and Benfotiamine can be retained, separated, and analyzed. This analysis method can be UV detected at 280 nm.
Column | Primesep S 4.6×150 mm, 5 µm, 100A |
Mobile Phase | MeCN/H2O -70/30%, |
Buffer | H3PO4 – 0.5% |
Flow Rate | 1.0 ml/min |
Detection | UV 280 nm |
Class of Compounds |
Vitamin, Drug |
Analyzing Compounds | Pyridoxine Hydrochloride, Benfotiamine |
Application Column
Primesep S
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsBenfotiamine
Pyridoxine hydrochloride
Vitamin B6 (Pyridoxine)
Separation of Pyridoxine hydrochloride on Newcrom R1 HPLC column
February 16, 2018
Pyridoxine hydrochloride can be analyzed by this reverse phase (RP) HPLC method with simple conditions. The mobile phase contains an acetonitrile (MeCN), water, and phosphoric acid. For Mass-Spec (MS) compatible applications the phosphoric acid needs to be replaced with formic acid. Smaller 3 µm particles columns available for fast UPLC applications. This liquid chromatography method is scalable and can be used for isolation impurities in preparative separation. It also suitable for pharmacokinetics.
Application Column
Newcrom R1
The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.
Select options