Separation type: Liquid Chromatography Mixed-mode
High Performance Liquid Chromatography (HPLC) Method for Analysis of Pyridoxine Hydrochloride (Vitamin B6) and Benfotiamine
Pyridoxine Hydrochloride (also known as Vitamin B6), is a naturally occurring vitamin that can be found in a wide variety of common foods, such as meat, grains, and avocados. Benfotiamine is a synthetic form of Vitamin B1 that has shown early signs of treating nerve damage, Alzheimer’s, alcohol dependence. They can both be found in Milgamma 100 tablets, a medicine prescribed to treat Vitamin B1 and B6 deficiency.
These two vitamins can be detected in the low UV regime. Using a Primesep S normal-phase column and a mobile phase consisting of water and acetonitrile (MeCN) with a phosphoric acid (H3PO4) buffer, Pyridoxine Hydrochloride and Benfotiamine can be retained, separated, and analyzed. This analysis method can be UV detected at 280 nm.
| Column | Primesep S 4.6×150 mm, 5 µm, 100A |
| Mobile Phase | MeCN/H2O -70/30%, |
| Buffer | H3PO4 – 0.5% |
| Flow Rate | 1.0 ml/min |
| Detection | UV 280 nm |
| Class of Compounds |
Vitamin, Drug |
| Analyzing Compounds | Pyridoxine Hydrochloride, Benfotiamine |
Application Column
Primesep S
The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.
Select optionsBenfotiamine
Pyridoxine hydrochloride
Vitamin B6 (Pyridoxine)
