HPLC Method for Analysis of Ascorbic Acid and Sodium Metabisulfite on Newcrom BH Column

HPLC Method for Analysis of Ascorbic Acid, Sodium metabisulfite, Sodium bisulfite on Newcrom BH by SIELC Technologies


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*The metabisulfite ion (S2O52−) is hydrolyzed to bisulfite (HSO3−) in water. Sodium metabisulfite is a white crystalline or powder solid. It has many uses, but some of it’s more prominent are: as the source of SO2 in wine, as a bleaching agent in the production of Coconut cream, and added to anesthetic solutions to prevent oxidation to improve the shelf life of the solution. Ascorbic is found naturally in citrus fruits and many vegetables. As a medication, it is used to prevent or treat low levels of vitamin C, since it is that vitamin. Vitamin C is needed to maintain the health of skin, cartilage, teeth, bone, and blood vessels. Ascorbic Acid and Sodium Metabisulfite can be separated, retained, and analyzed on a Newcrom BHmixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a phosphoric acid (H3PO $) buffer. This analysis method can be detected in the UV 275 nm.

Column(Column variation not found)
Mobile PhaseMeCN/H2O – 50/50%
BufferH3PO4 – 0.5%
Flow Rate0.5 ml/min
DetectionUV 275nm
Class of CompoundsAcid
Analyzing CompoundsAscorbic Acid, Sodium metabisulfite, Sodium bisulfite

High Performance Liquid Chromatography (HPLC) Method for Analysis of  Ascorbic Acid, Sodium metabisulfite, Sodium bisulfite

Application Column

Newcrom BH

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

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Primesep SB

The Primesep family of mixed-mode columns offers a wide variety of stationary phases, boasting unprecedented selectivity in the separation of a broad array of chemical compounds across multiple applications. Corresponding Primesep guard columns, available with all stationary phases, do not require holders. SIELC provides a method development service available to all customers. Inquire about our specially-tailored custom LC-phases for specific separations.

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Application Analytes:
Ascorbic Acid
Sodium bisulfite
Sodium metabisulfite
Application Detection:
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.