HPLC Method for Separation of Pyridoxine and Doxylamine on Obelisc R Column

HPLC Separation of Pyridoxine and Doxylamine on Obelisc R Column_1233


Column Obelisc R, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN/H2O – 30/70%
Buffer AmAc pH 4.0. gradient from 10 to 60 mM in 6 min, 4 min hold
Flow Rate 1.0 ml/min
Detection UV 270 nm,  MS- compatible mobile phase


Class of Compounds Hydrophobic, Drug
Analyzing Compounds Pyridoxine,  Doxylamine

Application Column

Obelisc R

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.

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Application Analytes:
Vitamin B6 (Pyridoxine)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.