HPLC Separation of Perfluoroalkyl Acids PFBA, PFAO, PEAS on Newcrom A Column

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Perfluoroalkyl acids (PFAAs) such as Perfluorobutyric acid, Perfluorononanoic acid and Perfluorooctylsulfonic acid are contaminants that have increasingly been found in groundwater and drinking water systems.
Perfluoroalkyl acids (PFAAs) are also strong surfactants that have been used industrially, in the production of fluoropolymers, but they are also present as residual impurities in a wide range of polymeric consumer products e.g. used to impart water and stain repellency in textiles, papers, and boards.
We were able to separate Perfluoroalkyl acids (PFAAs) in HPLC on Newcrom A mixed-mode column using a mobile phase of acetonitrile (ACN), and water with Ammonium Formate buffer. This separation can be monitored with ELSD, CAD detection techniques.


Column Newcom A, 4.6×150 mm, 5 µm, 100A
Mobile Phase MeCN – 40%
Buffer AmFm pH 3.0 – 50 mM
Flow Rate 1.0 ml/min
Detection CAD


Class of Compounds Perfluoroalkyl acids
Analyzing Compounds Perfluorobutyric acid, Perfluorononanoic acid, Perfluorooctylsulfonic acid


Application Column

Newcrom A

The Newcrom columns are a family of reverse-phase-based columns. Newcrom A, AH, B, and BH are all mixed-mode columns with either positive or negative ion-pairing groups attached to either short (25 Å) or long (100 Å) ligand chains. Newcrom R1 is a special reverse-phase column with low silanol activity.

Select options
Application Analytes:
Perfluorobutyric Acid (PFBA)
Perfluorooctanoic acid (PFOA)
Perfluorooctylsulfonic acid (PFAS)
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.