HPLC Method for Analysis of Cerium(III) Nitrate on Newcrom AH Column

HPLC Method for Separation of Cerium(III) nitrate hexahydrate on Newcrom AH by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode

HPLC Method for Analysis of Cerium(III) Nitrate on Newcrom AH Column by SIELC Technologies

Cerium(III) is a specific oxidation state of the element cerium, which is a lanthanide and a rare-earth metal.

Cerium(III) compounds are often used in a variety of applications. For example, cerium(III) chloride (CeCl3) is a common starting material for the preparation of other cerium-containing compounds and is often used as a reducing agent in organic chemistry. Cerium(III) oxide (Ce2O3), also known as cerous oxide, is used in ceramics and to polish glass.

Cerium(III) nitrate is often used in the laboratory as a cerium(III) source because it is relatively stable and easy to handle. It can be used in a variety of reactions, such as the preparation of other cerium compounds or as a catalyst or co-catalyst in organic reactions.

Cerium(III) Nitrate can be retained and analyzed on a mixed-mode Newcrom AH, 4.6 x 100 mm, 5 µm, 100 A column with a mobile phase consisting of water, Acetonitrile (MeCN), and Ammonium Formate. This analysis method can be detected with an Evaporative Light Scattering Detector (ELSD) or any other evaporative detection method (CAD, ESI-MS).

LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

High Performance Liquid Chromatography (HPLC) Method for Analysis of Cerium(III) nitrate hexahydrate


ColumnNewcrom AH, 4.6 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN -50-70%, 10 min
BufferGradient AmFm pH 3.0 – 30-60 mM, 10 min
Flow Rate1.0 ml/min
DetectionELSD, 50C
Peak Retention Time6.02 min
LOD0.2 ppm


Class of CompoundsMetal
Analyzing CompoundsCerium(III) nitrate hexahydrate

Application Column

Newcrom AH

Column Diameter: 4.6 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:
Carbonic acid, cerium(3+) salt (3:2)
Cerium(III) nitrate hexahydrate

Application Detection:
ELSD Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.