HPLC MS Method for Analysis of Procaine on Primesep 200 Column

High Performance Liquid Chromatography (HPLC) Method for Analysis of Procaine on Primesep 200 by SIELC Technologies

Separation type: Liquid Chromatography Mixed-mode SIELC Technologies

HPLC MS Method for Analysis of Procaine on Primesep 200 Column
HPLC Method for Analysis of Procaine on Primesep 100 Column by SIELC Technologies

 High Performance Liquid Chromatography (HPLC) Method for Analysis of Procaine

Procaine is a local anesthetic drug that belongs to the class of amino esters. It is often used to numb specific areas of the body during medical procedures or minor surgeries. Procaine works by blocking nerve signals in the body, leading to a temporary loss of sensation in the treated area.

Some common uses of procaine include dental procedures, minor skin surgeries, and as an ingredient in certain formulations for pain relief. It is often combined with other medications, such as benzyl alcohol, to enhance its stability and effectiveness.

Procaine can be retained and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 294 nm

ColumnPrimesep 200, 10 x 100 mm, 5 µm, 100 A
Mobile PhaseGradient MeCN 30-60%, 10 min
BufferAmFm pH 3.0  – 30 mM
Flow Rate0.2 ml/min
DetectionUV 294 nm, SIM + 237
Samples 0.02 mg/ml
Injection volume5 µl
LOD*200 ppb
* LOD was determined for this combination of instrument, method, and analyte, and it can vary from one laboratory to another even when the same general type of analysis is being performed.

Class of Compounds
Analyzing CompoundsProcaine

Application Column

Primesep 200

Column Diameter: 10 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A

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Application Analytes:

Application Detection:
LC MS Detection
UV Detection
SIELC Technologies usually develops more than one method for each compound. Therefore, this particular method may not be the best available method from our portfolio for your specific application. Before you decide to implement this method in your research, please send us an email to research@sielc.com so we can ensure you get optimal results for your compound/s of interest.