High Performance Liquid Chromatography (HPLC) Method for Analysis of Procaine on Primesep 200 by SIELC Technologies
Procaine is a local anesthetic drug with the chemical formula C13H20N2O2. It belongs to the amino ester group of local anesthetics. It is employed for local anesthesia, providing pain relief in various medical and dental settings. Procaine works by blocking nerve impulses and causing temporary loss of sensation in a specific area of the body. It is usually administered through injection to induce local anesthesia. You can find detailed UV spectra of Procainamide, Procaine and information about its various lambda maxima by visiting the following link.
Procaine can be retained and analyzed using a Primesep 200 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase comprising water, acetonitrile (MeCN), and ammonium formate as a buffer. This method allows for detection using UV at 294 nm
| Column | Primesep 200, 10 x 100 mm, 5 µm, 100 A, dual ended |
| Mobile Phase | Gradient MeCN 30-60%, 10 min |
| Buffer | AmFm pH 3.0 – 30 mM |
| Flow Rate | 0.2 ml/min |
| Detection | UV 294 nm, SIM + 237 |
| Samples | 0.02 mg/ml |
| Injection volume | 5 µl |
| LOD* | 200 ppb |
| Class of Compounds | Amide |
| Analyzing Compounds | Procaine |
Application Column
Primesep 200
Column Diameter: 10 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
Column options: dual ended
UV Detection
