HPLC Method for Analysis of Sodium metabisulfite on Primesep SB by SIELC Technologies
High Performance Liquid Chromatography (HPLC) Method for Analysis of Sodium metabisulfite
*The metabisulfite ion (S2O52−) is hydrolyzed to bisulfite (HSO3−) in water. Sodium metabisulfite is a white crystalline or powder solid. It has many uses, but some of it’s more prominent are: as the source of SO2 in wine, as a bleaching agent in the production of Coconut cream, and added to anesthetic solutions to prevent oxidation to improve the shelf life of the solution. Ascorbic is found naturally in citrus fruits and many vegetables. As a medication, it is used to prevent or treat low levels of vitamin C, since it is that vitamin. Vitamin C is needed to maintain the health of skin, cartilage, teeth, bone, and blood vessels. Ascorbic Acid and Sodium Metabisulfite can be separated, retained, and analyzed on a Primesep SBmixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a formic acid as a buffer. This analysis method can be detected in the UV 275 nm.
| Column | Primesep SB.M, 3.2 x 100 mm, 5 µm, 100 A |
| Mobile Phase | MeCN/H2O – 50/50% |
| Buffer | Formic acid – 1.0% |
| Flow Rate | 0.5 ml/min |
| Detection | UV, 275 nm, ESI SIM: [M-H]- 81 |
| Class of Compounds | Acid, Hydrophilic, Ionizable, Disinfectant, Antioxidant, Preservative agent. |
| Analyzing Compounds | Sodium metabisulfite |
Application Column
Primesep SB.M
Column Diameter: 3.2 mm
Column Length: 100 mm
Particle Size: 5 µm
Pore Size: 100 A
LC MS Detection
